Prusa Slicer causes stringing, S3D not  

Z banding

If my eyes are not failing me, I think you have Z banding too.  

Attach a 3MF project file so we can check your settings.

Save your current  3MF project file, zip it up, and attach it to a reply here so we can see your part & settings and give better recommendations. It's too hard to play 20 questions guessing otherwise.

With acceleration set to 0, still stringing

As S3D doesn't send any M204 commands to set acceleration, I disabled all acceleration settings in the print settings so it picks the firmware defaults (set all to 0), but stringing still occurs.:

Seems I have to dig deeper in the generated G-Code to see what S3D is doing differently....

Of course the retraction can be well seen before a move, also when it resets the retraction.

Attached the project file....

Copying S3D settings verbatim doesn't always work out

Posted by: @davorin

As S3D doesn't send any M204 commands to set acceleration, I disabled all acceleration settings in the print settings so it picks the firmware defaults (set all to 0), but stringing still occurs.

Be sure to inspect your S3D gcode. Back when I used it, many of the "magic" profiles had M20x commands embedded in the start gcode.

Of course the retraction can be well seen before a move, also when it resets the retraction.

Are you using a Bowden extruder setup? You've got retraction set to 1.2mm which is low by bowden standards. Is it the same on your S3D setup?

Your extrusion multiplier is set to 1.029. If you've calibrated this value, not a problem, but be sure you really want it higher. I find most my stringing issues not caused by filament moisture are due to a small amount of over-extrusion. Be sure to calibrate your extrusion multiplier using PrusaSlicer, not just copying settings over from S3D. They work differently internally, and I found it much easier to create a new preset from scratch rather than trying to "save time" by copying S3D profile settings over.

I'm sure (o;

There is no M204 inside S3Ds generated G-Code...sublime text and grep is good at searching (o;

me@blender:~/Documents/FreeCad$ grep -c M204 Stringing*.gcode
Stringing_Test_Prusa.gcode:864
Stringing_Test_S3D.gcode:0

I added my printer config in the signature by intention to answer those questions by itself (o;  so yes..both my printers have a direct drive extruder...also the extrusion multiplier was calibrated...and I was surprised it was higher than in S3D....as I had before 0.96...but still with the stringing...

What makes me think is that, if PS had a general problem with stringing, more people would have shout about it...but didn't find anything related to my case....also tried quickly with Super Slicer, same results.....so I have to inspect what the printers klipper firmware might actually doing wrong here with the PS G-Code sequence....

I suspect the "reset extruder distance" difference might be it....just a guess...but I also tried with PA shut off in S3D and PS, no difference...

Have to flash this board with Marlin 2.0.9.x and see how it behaves then with S3D and PS G-Code....and get another one in parallel for klipper testing....